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tlrl-pic-5 Poly(I:C) (HMW) 50 mg重慶市華雅干細胞

簡要描述:Poly(I:C) HMW
Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA), a molecular pattern associated with viral infection.

  • 產(chǎn)品型號:tlrl-pic-5
  • 廠商性質(zhì):代理商
  • 更新時間:2017-05-04
  • 訪  問  量:2365
詳細介紹
品牌Invivogen

Poly(I:C) HMW

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Poly(I:C) is recognized by TLR3 inducing the activation of NF-kB and the production of cytokines.

Poly(I:C) is composed of a strand of poly(I) annealed to a strand of poly(C). The size of the strands varies. InvivoGen is the only company to provide poly(I:C) with two different sizes:
 - Poly(I:C) (HMW) with a high molecular weight has an average size of 1.5-8 kb.
 - Poly(I:C) (LMW) with a low molecular weight has an average size of 0.2-1 kb

Poly(I:C) is also available on ready-to-use 8-well strips pre-coated at 7 different concentrations and a negative control.
Specifications
CAS number: 31852-29-6

Working concentration: 10 ng - 10 μg/ml


Endotoxin level:  <0.001 EU/μg

Solubility: 5 mg/ml in physiological water (NaCl 0.9%) heated for 10 minutes at 65 - 70°C
Contents:
• Poly(I:C) is provided lyophilized and is available in two quantities:

- 10 mg: (catalog # tlrl-pic)
 - 50 mg: (catalog # tlrl-pic-5)

• Sterile endotoxin-free physiological water (NaCl 0.9%), 10 ml is provided with catalog #tlrl-pic and 2 x 25 ml with catalog #tlrl-pic-5

Poly(I:C) is shipped at room temperature and should be stored at 4°C. Upon resuspension, prepare aliquots of Poly(I:C) and store at -20°C for long term storage. Store at 4°C for short term storage. Lyophilized product is stable 1 year at 4°C when properly stored. Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.
Description:
Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2]. Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-β (TRIF, also known as TICAM-1) [3]. Activation of IRF3 leads to the production of type I IFNs, especially IFN-β. A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-κB and AP-1 [4]. Activation of this pathway triggers the production of inflammatory cytokines and chemokines such as TNF-α, IL-6 and CXCL10. Poly(I:C) is also recognized by the cytosolic RNA helicases retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associate gene 5 (MDA-5) [5].

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NFkappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double- stranded RNA-mediated signaling. BBRC, 293(5):1364-9.
3. Yamamoto M. et al., 2003. Role of Adaptor TRIF in the MyD88-Independent Toll-Like Receptor Signaling Pathway. Science 301: 640.
4. Kawai T & Akira S., 2008. Tolllikereceptor and RIG-I-like receptor signaling. Ann N Y Acad Sci. 1143:1-20. Review.
5. Kato H. et al., 2006. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 441(7089):101-5.

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